CHARACTERIZATION OF METHICILLIN - RESISTANT STAPHYLOCOCCUS AUREUS FROM ORTHOPAEDIC PATIENTS IN UNIVERSITY TEACHING HOSPITAL IN NIGERIA
- Project Research
- 1-5 Chapters
- Abstract : Available
- Table of Content: Available
- Reference Style: APA
- Recommended for : Student Researchers
- NGN 3000
ABSTRACT
Methicillin resistant Staphylococcus aureus (MRSA) is now a threat to both the hospitalized patients and community globally. This work was aimed at detecting molecularly, methicillin resistant Staphylococcus aureus from the orthopaedic patients in Ahmadu Bello University Teaching Hospital, Zaria, Nigeria. Conventional biochemical methods were used to identify the isolates while API STAPH identification test kit further characterized the isolates to species level. The susceptibility test was carried out using disc agar diffusion method while beta – lactamase production was tested for using nitrocefin. Methicillin resistance was detected phenotypically using cefoxitin 30 µg disc and oxacillin agar screen test. Multiplex polymerase chain reaction (PCR) was used to detect mecA gene, the gene coding methicillin resistance and blaZ gene, the gene coding for beta- lactamase production with 16SrRNA gene being the internal control. Sequencing was carried out for the amplified isolates. A total number of 126 samples were collected from wound, skin and bed of orthopaedic patients for 5 months. With the conventional biochemical method of identification, 100(79.4%) isolates were identified as S. aureus while with the use of API STAPH identification test kit 39(39%) of the 100 were characterized as S. aureus. The susceptibility test of the S. aureus isolates showed that gentamicin had the greatest activity: 100%, 100% and 93.8% in wound, bed and skin respectively, followed by ciprofloxacin (100%, 94.1% and 93.8%) and pefloxacin (100%, 88.2% and 75%) respectively. However, the greatest level of resistance was observed with ampicillin: 100%, 100% and 87.5% in wound, bed and skin respectively followed by ceftriaxone: 100%, 76.5% and 75%; and amoxicillin - clavulanate: 66.7%, 58.8%, 56.2%. Phenotypic detection of MRSA with the use of cefoxitin disc diffusion gave a MRSA prevalence of 83.3%, 64.5% and 56.3% from wound, bed and skin viii respectively. These MRSA isolates were generally resistant to the beta lactam antibiotics used, while 11/25 (44%) were multi-drug resistant. However, vancomycin, gentamicin and ciprofloxacin were most active against the MRSA isolates, 15% of these phenotypic MRSA isolates were hyper-producers of beta-lactamase. The gold standard for detecting MRSA using polymerase chain reaction is detection of mecA gene and only 2 (5.1%) S. aureus isolates were positive. Fifteen (78.9%) of the phenotypic MRSA tested carried plasmid with molecular weight ranging from 9.23 to 13.27 kilobase pairs. The presence of plasmid and hyper- production of betalactamase can be suggested to be responsible for the phenotypic detection of MRSA observed in this study; 33.3% of the S. aureus isolates amplified blaZ gene. The nucleotide sequence of 16SrRNA gene of isolate S41 in comparison with those from GenBank database showed that the S. aureus isolate has 99% identity with Staphylococcus aureus strain KIBGE-MB01 with sequence ID (accession) number HM061132.1.
Related Project Materials
A SURVEY ON PERSONAL AND ENVIRONMENTAL HYGIENE IN THE TRANSMISSION OF COMMUNICABLE DISEASES
BACKGROUND OF THE STUDY
Akerman (2001) defines personal hygiene as "practices that an individual e...
PHYTOCHEMICAL AND ANTIBACTERIAL STUDIES OF Ensete gilletii ROOT EXTRACTS
ABSTRACT
Ensete gilletii commonly called wild banana, is ethno-medicinally used for the treatment of diarrhea, dysentery, typhoid, stomac...
A SURVEY RESEARCH TO EXAMINE THE EFFECTS OF FEMALE GENITAL MUTILATION: A CASE STUDY OF BENIN CITY, EDO STATE, NIGERIA
BACKGROUND OF THE STUDY
Female Genital Mutilation (FGM) also known as Female Genital Cutting (FGC), Female circumcision,...
THE IMPACT OF ADVERTISING ON CUSTOMER PATRONAGE
BACKGROUND OF THE STUDY
Advertising is one of major ways in which sellers use in order to stimula...
DESIGN AND IMPLEMENTATION OF CAR RENTAL AND TRACKING SYSTEM
Statement of the Problem
In an organization running a car rental service, the process for searching client details is sl...
CAUSES AND EFFECTS OF COMMUNICATION BREAKDOWN IN AN ORGANIZATION (A case study of champions breweries Uyo Akwa Ibom State)
ABSTRACT
The study intends to determine the importance of communication in enhancing an effective management underta...
INFLUENCE OF SECOND CHANCE (A FOREIGN TELEVISION PROGRAMME ON THE SOCIAL BEHAVIOUR OF NNAMDI AZIKIWE UNIVERSITY STUDENTS (AWKA)
Background of the study
Technological advancement in communication technologies has continued to eulogize television as a powerful medium...
THE INFLUENCE OF TELEVISION EDUCATIONAL AND ENTERTAINMENT PROGRAMMES ON VIEWERS
ABSTRACT
This study was carried out on the influence of television educational and entertainment progra...
TEACHERS’ MOTIVATION AS A TOOL FOR EFFECTIVE SECONDARY SCHOOL ADMINISTRATION
ABSTRACT
Motivation represents an employee’s desire and commitment, which is manifested as job performance. That...
THE IMPACT OF DEBT BURDEN ON ECONOMIC GROWTH OF NIGERIA
ABSTRACT
The study investigated the impact of external debt on economic growth in Nigeria for the p...
